Processing time, protein recovery, and pH accuracy are important aspects of evaluating buffer exchange, but it is also crucial to study the impact of each buffer exchange process on protein stability.

The buffer composition was shown to have no influence on the quantification results for the buffers tested (Figure). In each case, the concentration of the spiked protein was correctly determined.

Buffer for protein resuspension: Several possible buffers can be used to resuspend the proteins; the buffer of choice will depend on how the proteins will be analyzed. Two common buffers are ...

Protein preparation for NMR gives further details on how to optimise the buffer conditions for your sample. As for peptides, protein spectra can be acquired at concentrations >0.1mM for a 1D or 2D 15 ...

Protein crystallization is an important tool to purify proteins as well as to demonstrate their chemical purity.

The protein buffer was Tris 20 mM, pH 7.5. d, We examined the dipeptidase fibre-forming mutant fused to YFP by TEM with negative straining. The protein buffer was Tris 20 mM, 100 mM NaCl, pH 7.5.

The buffer is compatible with most downstream applications, including chromatography, electrophoresis, enzyme assays, kinase assays and immunoassays. The Yeast Protein Extraction Buffer Kit is ...